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Attention, votre centre de documentation sera fermé du 27/04 au 12/05 inclus.
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Auteur Luc Beaugnet |
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How temperature affects fertility in the key pollinator Bombus terrestris / Luc Beaugnet
Titre : How temperature affects fertility in the key pollinator Bombus terrestris Type de document : TFE / Mémoire Auteurs : Luc Beaugnet, Auteur ; Seth Barribeau, Directeur de la recherche Editeur : Montignies-sur-Sambre : Helha. Paramédical - Biologie médicale Année de publication : 2020 Note générale : Le fichier numérique de ce document est disponible uniquement pour les membres de la Haute Ecole Louvain-en-Hainaut ainsi que ses étudiants. Veuillez vous connecter pour accéder à votre compte lecteur. Langues : Français (fre) Mots-clés : biologie médicale Index. décimale : TFE Bio Med TFE Biologie médicale Résumé : Pollinators such as bumblebees are key species in various fields including ecosystem conservation and agriculture. In recent years, mainly linked to global warming but also to many harmful human activities, these insects are facing serious threats of extinction or migration. It is therefore necessary to collect as much information as possible in order to predict the future of these species as effectively as possible.
Through this project, a very specific parameter of an important and widespread bumblebee species (Bombus terrestris) wanted to be studied, which is the impact of sub-lethal temperature on male fertility. Males are directly affected by high summer temperatures during their breeding period. They are therefore in the front line of the global warming.
In order to determine the influence of temperature on their fertility, it was necessary to go through different stages starting with the breeding of male bumblebees, through the determination of the maximum critical temperature and the fertility tests after stress. As the project was forced to end prematurely, the steps that could not be carried out were presented in connection with other similar studies. The results obtained combined with the results of other studies made it possible to formulate hypotheses concerning future applications and research prospects.Note de contenu : Table of content
Presentation of the University laboratory............................................................................. 5
General introduction ............................................................................................................. 7
1. Theoretical background ................................................................................................. 9
a. Family ..................................................................................................................................... 9
b. Genus Bombus ....................................................................................................................... 9
c. Life cycle ................................................................................................................................. 9
d. Physical traits and thermoregulation ................................................................................... 11
e. Geographic distribution ....................................................................................................... 13
2. Objectives ..................................................................................................................... 14
3. Research strategy ......................................................................................................... 15
a. Bee husbandry ...................................................................................................................... 16
b. Definition of the experimental conditions ........................................................................... 16
c. Fertility ................................................................................................................................. 17
i. The state of the genitals ................................................................................................... 18
ii. Spermatozoa viability ....................................................................................................... 18
iii. Data analysis ..................................................................................................................... 19
4. Material and methods .................................................................................................. 20
a. Bee husbandry ...................................................................................................................... 20
b. Critical temperature range determination ........................................................................... 22
c. Dissection and sperm viability measurement ...................................................................... 23
i. Motility ............................................................................................................................. 24
ii. Live/dead staining coupled with fluorescence microscopy or flow cytometry ............... 25
d. Data analysis ......................................................................................................................... 28
5. First results ................................................................................................................... 29
6. Discussion and outlook ................................................................................................ 31
Conclusion ........................................................................................................................... 35
References ........................................................................................................................... 36Permalink : ./index.php?lvl=notice_display&id=89140 How temperature affects fertility in the key pollinator Bombus terrestris [TFE / Mémoire] / Luc Beaugnet, Auteur ; Seth Barribeau, Directeur de la recherche . - Montignies-sur-Sambre : Helha. Paramédical - Biologie médicale, 2020.
Le fichier numérique de ce document est disponible uniquement pour les membres de la Haute Ecole Louvain-en-Hainaut ainsi que ses étudiants. Veuillez vous connecter pour accéder à votre compte lecteur.
Langues : Français (fre)
Mots-clés : biologie médicale Index. décimale : TFE Bio Med TFE Biologie médicale Résumé : Pollinators such as bumblebees are key species in various fields including ecosystem conservation and agriculture. In recent years, mainly linked to global warming but also to many harmful human activities, these insects are facing serious threats of extinction or migration. It is therefore necessary to collect as much information as possible in order to predict the future of these species as effectively as possible.
Through this project, a very specific parameter of an important and widespread bumblebee species (Bombus terrestris) wanted to be studied, which is the impact of sub-lethal temperature on male fertility. Males are directly affected by high summer temperatures during their breeding period. They are therefore in the front line of the global warming.
In order to determine the influence of temperature on their fertility, it was necessary to go through different stages starting with the breeding of male bumblebees, through the determination of the maximum critical temperature and the fertility tests after stress. As the project was forced to end prematurely, the steps that could not be carried out were presented in connection with other similar studies. The results obtained combined with the results of other studies made it possible to formulate hypotheses concerning future applications and research prospects.Note de contenu : Table of content
Presentation of the University laboratory............................................................................. 5
General introduction ............................................................................................................. 7
1. Theoretical background ................................................................................................. 9
a. Family ..................................................................................................................................... 9
b. Genus Bombus ....................................................................................................................... 9
c. Life cycle ................................................................................................................................. 9
d. Physical traits and thermoregulation ................................................................................... 11
e. Geographic distribution ....................................................................................................... 13
2. Objectives ..................................................................................................................... 14
3. Research strategy ......................................................................................................... 15
a. Bee husbandry ...................................................................................................................... 16
b. Definition of the experimental conditions ........................................................................... 16
c. Fertility ................................................................................................................................. 17
i. The state of the genitals ................................................................................................... 18
ii. Spermatozoa viability ....................................................................................................... 18
iii. Data analysis ..................................................................................................................... 19
4. Material and methods .................................................................................................. 20
a. Bee husbandry ...................................................................................................................... 20
b. Critical temperature range determination ........................................................................... 22
c. Dissection and sperm viability measurement ...................................................................... 23
i. Motility ............................................................................................................................. 24
ii. Live/dead staining coupled with fluorescence microscopy or flow cytometry ............... 25
d. Data analysis ......................................................................................................................... 28
5. First results ................................................................................................................... 29
6. Discussion and outlook ................................................................................................ 31
Conclusion ........................................................................................................................... 35
References ........................................................................................................................... 36Permalink : ./index.php?lvl=notice_display&id=89140 Exemplaires
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