[article] 
inLabAnimal-Europe > 7/10 (Juillet 2010) . - p. 34-38

Titre :	Multiple ovarian transplants to rescue a transgenic line of mice.
Type de document :	texte imprimé
Auteurs :	Joyce Dawes
Année de publication :	2010
Article en page(s) :	p. 34-38
Langues :	Anglais (eng)
Résumé :	Transgenic mice are useful tools for studying gene function and regulation but can be difficult to successfully breed. To 'rescue' transgenic lines that are difficult to propagate, researchers use a variety of techniques. One method is ovarian transplant, in which researchers remove ovaries from a donor transgenic mouse, cryopreserve the ovarian tissue, transplant this tissue into histocompatible female mice and breed these recipient females. Though it is a useful technique, cryopreservation can potentially damage ovarian tissue, which could reduce fertility. In this article, the authors describe how they carried out ovarian transplants without cryopreservation to rescue a line of transgenic C57BL/6 mice. Other researchers who have experience with mouse reproductive surgery should be able to use this technique to rescue infertile transgenic lines of mice.
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[article] Multiple ovarian transplants to rescue a transgenic line of mice. [texte imprimé] / Joyce Dawes . - 2010 . - p. 34-38.
Langues : Anglais (eng)
in LabAnimal-Europe > 7/10 (Juillet 2010) . - p. 34-38

Résumé :

Transgenic mice are useful tools for studying gene function and regulation but can be difficult to successfully breed. To 'rescue' transgenic lines that are difficult to propagate, researchers use a variety of techniques. One method is ovarian transplant, in which researchers remove ovaries from a donor transgenic mouse, cryopreserve the ovarian tissue, transplant this tissue into histocompatible female mice and breed these recipient females. Though it is a useful technique, cryopreservation can potentially damage ovarian tissue, which could reduce fertility. In this article, the authors describe how they carried out ovarian transplants without cryopreservation to rescue a line of transgenic C57BL/6 mice. Other researchers who have experience with mouse reproductive surgery should be able to use this technique to rescue infertile transgenic lines of mice.

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