Impact of test chamber design on spontaneous behavioral responses of model crustacean zooplankton Artemia franciscana / Jason Henry in LabAnimal, Vol.22 N°4 (avril 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°4 (avril 2022) . - p. 8-17 Titre : Impact of test chamber design on spontaneous behavioral responses of model crustacean zooplankton Artemia franciscana Type de document : texte imprimé Auteurs : Jason Henry ; Yutao Bai ; Daniel Williams ; Adrian Logozzo ; Alex Ford ; Donald Wlodkowic Année de publication : 2022 Article en page(s) : p. 8-17 Note générale : https://doi.org/10.1038/s41684-021-00908-7 Langues : Anglais (eng) Mots-clés : Behavioural ecology  Behavioural methods Résumé : The use of small aquatic model organisms to investigate the behavioral effects of chemical exposure is becoming an integral component of aquatic ecotoxicology research and neuroactive drug discovery. Despite the increasing use of invertebrates for behavioral phenotyping in toxicological studies and chemical risk assessments, little is known regarding the potential for environmental factors—such as geometry, size, opacity and depth of test chambers—to modulate common behavioral responses. In this work, we demonstrate that test chamber geometry, size, opacity and depth can affect spontaneous, unstimulated behavioral responses of euryhaline crustacean Artemia franciscana first instar larval stages. We found that in the absence of any obvious directional cues, A. franciscana exhibited a strong innate wall preference behavior. Using different test chamber sizes and geometries, we found both increased wall preference and lowered overall distance traveled by the test shrimp in a smaller chamber with sharper-angled vertices. It was also determined through quantifiable changes in the chambers’ color that the A. franciscana early larval stages can perceive, differentiate and react to differences in color or perhaps rather to light transmittance of the test chambers. The interaction between innate edge preference and positive phototaxis could be consistently altered with a novel photic stimulus system. We also observed a strong initial preference for depth in A. franciscana first instar larval stages, which diminished through the acclimatization. We postulate that the impact of test chamber designs on neurobehavioral baseline responses warrants further investigation, in particular considering the increased interest in behavioral eco-neurotoxicology applications. Permalink : ./index.php?lvl=notice_display&id=102720 [article] Impact of test chamber design on spontaneous behavioral responses of model crustacean zooplankton Artemia franciscana [texte imprimé] / Jason Henry ; Yutao Bai ; Daniel Williams ; Adrian Logozzo ; Alex Ford ; Donald Wlodkowic . - 2022 . - p. 8-17. https://doi.org/10.1038/s41684-021-00908-7 Langues : Anglais (eng) in LabAnimal > Vol.22 N°4 (avril 2022) . - p. 8-17 Mots-clés : Behavioural ecology  Behavioural methods Résumé : The use of small aquatic model organisms to investigate the behavioral effects of chemical exposure is becoming an integral component of aquatic ecotoxicology research and neuroactive drug discovery. Despite the increasing use of invertebrates for behavioral phenotyping in toxicological studies and chemical risk assessments, little is known regarding the potential for environmental factors—such as geometry, size, opacity and depth of test chambers—to modulate common behavioral responses. In this work, we demonstrate that test chamber geometry, size, opacity and depth can affect spontaneous, unstimulated behavioral responses of euryhaline crustacean Artemia franciscana first instar larval stages. We found that in the absence of any obvious directional cues, A. franciscana exhibited a strong innate wall preference behavior. Using different test chamber sizes and geometries, we found both increased wall preference and lowered overall distance traveled by the test shrimp in a smaller chamber with sharper-angled vertices. It was also determined through quantifiable changes in the chambers’ color that the A. franciscana early larval stages can perceive, differentiate and react to differences in color or perhaps rather to light transmittance of the test chambers. The interaction between innate edge preference and positive phototaxis could be consistently altered with a novel photic stimulus system. We also observed a strong initial preference for depth in A. franciscana first instar larval stages, which diminished through the acclimatization. We postulate that the impact of test chamber designs on neurobehavioral baseline responses warrants further investigation, in particular considering the increased interest in behavioral eco-neurotoxicology applications. Permalink : ./index.php?lvl=notice_display&id=102720

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A review of 28 free animal-tracking software applications: current features and limitations / Veronica Panadeiro in LabAnimal, Vol. 21 N°10 (October 2021)

Public ISBD [article]  inLabAnimal > Vol. 21 N°10 (October 2021) . - p. 16-31 Titre : A review of 28 free animal-tracking software applications: current features and limitations Type de document : texte imprimé Auteurs : Veronica Panadeiro ; Alvaro Rodriguez ; Jason Henry ; Donald Wlodkowic ; Magnus Andersson Année de publication : 2021 Article en page(s) : p. 16-31 Note générale : DOI: 10.1038/s41684-021-00811-1 Langues : Anglais (eng) Mots-clés : Algorithms  Animals  Behavior, Animal  Software Résumé : Well-quantified laboratory studies can provide a fundamental understanding of animal behavior in ecology, ethology and ecotoxicology research. These types of studies require observation and tracking of each animal in well-controlled and defined arenas, often for long timescales. Thus, these experiments produce long time series and a vast amount of data that require the use of software applications to automate the analysis and reduce manual annotation. In this review, we examine 28 free software applications for animal tracking to guide researchers in selecting the software that might best suit a particular experiment. We also review the algorithms in the tracking pipeline of the applications, explain how specific techniques can fit different experiments, and finally, expose each approach's weaknesses and strengths. Our in-depth review includes last update, type of platform, user-friendliness, off- or online video acquisition, calibration method, background subtraction and segmentation method, species, multiple arenas, multiple animals, identity preservation, manual identity correction, data analysis and extra features. We found, for example, that out of 28 programs, only 3 include a calibration algorithm to reduce image distortion and perspective problems that affect accuracy and can result in substantial errors when analyzing trajectories and extracting mobility or explored distance. In addition, only 4 programs can directly export in-depth tracking and analysis metrics, only 5 are suited for tracking multiple unmarked animals for more than a few seconds and only 11 have been updated in the period 2019-2021. Permalink : ./index.php?lvl=notice_display&id=96698 [article] A review of 28 free animal-tracking software applications: current features and limitations [texte imprimé] / Veronica Panadeiro ; Alvaro Rodriguez ; Jason Henry ; Donald Wlodkowic ; Magnus Andersson . - 2021 . - p. 16-31. DOI: 10.1038/s41684-021-00811-1 Langues : Anglais (eng) in LabAnimal > Vol. 21 N°10 (October 2021) . - p. 16-31 Mots-clés : Algorithms  Animals  Behavior, Animal  Software Résumé : Well-quantified laboratory studies can provide a fundamental understanding of animal behavior in ecology, ethology and ecotoxicology research. These types of studies require observation and tracking of each animal in well-controlled and defined arenas, often for long timescales. Thus, these experiments produce long time series and a vast amount of data that require the use of software applications to automate the analysis and reduce manual annotation. In this review, we examine 28 free software applications for animal tracking to guide researchers in selecting the software that might best suit a particular experiment. We also review the algorithms in the tracking pipeline of the applications, explain how specific techniques can fit different experiments, and finally, expose each approach's weaknesses and strengths. Our in-depth review includes last update, type of platform, user-friendliness, off- or online video acquisition, calibration method, background subtraction and segmentation method, species, multiple arenas, multiple animals, identity preservation, manual identity correction, data analysis and extra features. We found, for example, that out of 28 programs, only 3 include a calibration algorithm to reduce image distortion and perspective problems that affect accuracy and can result in substantial errors when analyzing trajectories and extracting mobility or explored distance. In addition, only 4 programs can directly export in-depth tracking and analysis metrics, only 5 are suited for tracking multiple unmarked animals for more than a few seconds and only 11 have been updated in the period 2019-2021. Permalink : ./index.php?lvl=notice_display&id=96698

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