Multiple ovarian transplants to rescue a transgenic line of mice. / Joyce Dawes in LabAnimal-Europe, 7/10 (Juillet 2010)

Public ISBD [article]  inLabAnimal-Europe > 7/10 (Juillet 2010) . - p. 34-38 Titre : Multiple ovarian transplants to rescue a transgenic line of mice. Type de document : texte imprimé Auteurs : Joyce Dawes Année de publication : 2010 Article en page(s) : p. 34-38 Langues : Anglais (eng) Résumé : Transgenic mice are useful tools for studying gene function and regulation but can be difficult to successfully breed. To 'rescue' transgenic lines that are difficult to propagate, researchers use a variety of techniques. One method is ovarian transplant, in which researchers remove ovaries from a donor transgenic mouse, cryopreserve the ovarian tissue, transplant this tissue into histocompatible female mice and breed these recipient females. Though it is a useful technique, cryopreservation can potentially damage ovarian tissue, which could reduce fertility. In this article, the authors describe how they carried out ovarian transplants without cryopreservation to rescue a line of transgenic C57BL/6 mice. Other researchers who have experience with mouse reproductive surgery should be able to use this technique to rescue infertile transgenic lines of mice. Permalink : ./index.php?lvl=notice_display&id=76490 [article] Multiple ovarian transplants to rescue a transgenic line of mice. [texte imprimé] / Joyce Dawes . - 2010 . - p. 34-38. Langues : Anglais (eng) in LabAnimal-Europe > 7/10 (Juillet 2010) . - p. 34-38 Résumé : Transgenic mice are useful tools for studying gene function and regulation but can be difficult to successfully breed. To 'rescue' transgenic lines that are difficult to propagate, researchers use a variety of techniques. One method is ovarian transplant, in which researchers remove ovaries from a donor transgenic mouse, cryopreserve the ovarian tissue, transplant this tissue into histocompatible female mice and breed these recipient females. Though it is a useful technique, cryopreservation can potentially damage ovarian tissue, which could reduce fertility. In this article, the authors describe how they carried out ovarian transplants without cryopreservation to rescue a line of transgenic C57BL/6 mice. Other researchers who have experience with mouse reproductive surgery should be able to use this technique to rescue infertile transgenic lines of mice. Permalink : ./index.php?lvl=notice_display&id=76490

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