Refining timed pregnancies in two strains of genetically engineered mice. / Sarah L. Mader in LabAnimal-Europe, 10/09 (Octobre 2009)

Public ISBD [article]  inLabAnimal-Europe > 10/09 (Octobre 2009) . - p. 20-30 Titre : Refining timed pregnancies in two strains of genetically engineered mice. Type de document : texte imprimé Auteurs : Sarah L. Mader, Auteur Année de publication : 2009 Article en page(s) : p. 20-30 Langues : Anglais (eng) Résumé : In order to efficiently generate genetically engineered mouse (GEM) fetuses or neonates of a specified age range, researchers must develop strain-specific strategies, including reliable early pregnancy detection. The authors evaluated pregnancy indices (pregnancy rate, plug rate, pregnant plugged rate, first litter size and body weight) in two GEM breeding colonies: homozygous soluble epoxide hydrolase knockout (sEHKO) mice (n=164 females) and L7-tau-green fluorescent protein (GFP) transgenic mice (n=61 females). The goals of the study were to determine the most accurate early pregnancy indicator and to reliably and cost-effectively produce timed pregnant females that were between gestation days 16 and 18. The authors set up each timed mating by placing two naturally synchronized females with a male for 48 h. When males were present, personnel checked each female daily for a vaginal plug. They then weighed the females immediately, 1 week and 2 weeks after removing the males. In both sEHKO and GFP colonies, increases in body weight at 1 and 2 weeks after timed male exposure more reliably and consistently indicated pregnancy than did plug detection. Further evaluations and protocol refinements are planned based on litter size and litter number in these colonies. Permalink : ./index.php?lvl=notice_display&id=76457 [article] Refining timed pregnancies in two strains of genetically engineered mice. [texte imprimé] / Sarah L. Mader, Auteur . - 2009 . - p. 20-30. Langues : Anglais (eng) in LabAnimal-Europe > 10/09 (Octobre 2009) . - p. 20-30 Résumé : In order to efficiently generate genetically engineered mouse (GEM) fetuses or neonates of a specified age range, researchers must develop strain-specific strategies, including reliable early pregnancy detection. The authors evaluated pregnancy indices (pregnancy rate, plug rate, pregnant plugged rate, first litter size and body weight) in two GEM breeding colonies: homozygous soluble epoxide hydrolase knockout (sEHKO) mice (n=164 females) and L7-tau-green fluorescent protein (GFP) transgenic mice (n=61 females). The goals of the study were to determine the most accurate early pregnancy indicator and to reliably and cost-effectively produce timed pregnant females that were between gestation days 16 and 18. The authors set up each timed mating by placing two naturally synchronized females with a male for 48 h. When males were present, personnel checked each female daily for a vaginal plug. They then weighed the females immediately, 1 week and 2 weeks after removing the males. In both sEHKO and GFP colonies, increases in body weight at 1 and 2 weeks after timed male exposure more reliably and consistently indicated pregnancy than did plug detection. Further evaluations and protocol refinements are planned based on litter size and litter number in these colonies. Permalink : ./index.php?lvl=notice_display&id=76457

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