Dépouillements

Expanded precision genome-editing toolbox for human disease modeling in zebrafish / Pratishtha Varshney in LabAnimal, Vol.22 N°12 (décembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°12 (décembre 2022) . - p. 21-22 Titre : Expanded precision genome-editing toolbox for human disease modeling in zebrafish Type de document : texte imprimé Auteurs : Pratishtha Varshney ; Gaurav K. Varshney Année de publication : 2022 Article en page(s) : p. 21-22 Langues : Anglais (eng) Mots-clés : biotechnologie  génétique Résumé : The application of genome-editing tools to generate point mutations in animal models is of particular value for precise disease modeling, but the PAM requirement of Cas enzymes is a critical limiting factor. Two new studies demonstrate that SpRY variant displays efficient genome editing in a nearly PAM-less manner in zebrafish, expanding the targeting scope of base editors in this model. Permalink : ./index.php?lvl=notice_display&id=107929 [article] Expanded precision genome-editing toolbox for human disease modeling in zebrafish [texte imprimé] / Pratishtha Varshney ; Gaurav K. Varshney . - 2022 . - p. 21-22. Langues : Anglais (eng) in LabAnimal > Vol.22 N°12 (décembre 2022) . - p. 21-22 Mots-clés : biotechnologie  génétique Résumé : The application of genome-editing tools to generate point mutations in animal models is of particular value for precise disease modeling, but the PAM requirement of Cas enzymes is a critical limiting factor. Two new studies demonstrate that SpRY variant displays efficient genome editing in a nearly PAM-less manner in zebrafish, expanding the targeting scope of base editors in this model. Permalink : ./index.php?lvl=notice_display&id=107929

Réservation

Réserver ce document

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Disponible Disponible

From Rats to Humans: how novel behavioral paradigms and reinforcement learning can bridge the gap in translation / Alexander Gonzalez in LabAnimal, Vol.22 N°12 (décembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°12 (décembre 2022) . - p. 23-24 Titre : From Rats to Humans: how novel behavioral paradigms and reinforcement learning can bridge the gap in translation Type de document : texte imprimé Auteurs : Alexander Gonzalez ; Lisa M. Giocomo Année de publication : 2022 Article en page(s) : p. 23-24 Langues : Anglais (eng) Mots-clés : technique biologique  neuroscience Résumé : A challenge in translational research is comparing behaviors across species. A new study combines a novel behavioral paradigm in rats and humans with reinforcement learning to infer shared computations for goal-directed navigation. Permalink : ./index.php?lvl=notice_display&id=107930 [article] From Rats to Humans: how novel behavioral paradigms and reinforcement learning can bridge the gap in translation [texte imprimé] / Alexander Gonzalez ; Lisa M. Giocomo . - 2022 . - p. 23-24. Langues : Anglais (eng) in LabAnimal > Vol.22 N°12 (décembre 2022) . - p. 23-24 Mots-clés : technique biologique  neuroscience Résumé : A challenge in translational research is comparing behaviors across species. A new study combines a novel behavioral paradigm in rats and humans with reinforcement learning to infer shared computations for goal-directed navigation. Permalink : ./index.php?lvl=notice_display&id=107930

Réservation

Réserver ce document

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Disponible Disponible

Opportunity for Improvement / Christina Nascimento in LabAnimal, Vol.22 N°12 (décembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°12 (décembre 2022) . - p. 19-20 Titre : Opportunity for Improvement Type de document : texte imprimé Auteurs : Christina Nascimento Année de publication : 2022 Article en page(s) : p. 19-20 Langues : Anglais (eng) Mots-clés : communauté scientifique  politique Résumé : This scenario provides several areas for process improvement for the IACUC, researcher and Safety Officer. The use of containment (assumed, biocontainment) may be excessive burden for a commonly used hazardous chemical agent such as tamoxifen. The Principal Investigator (PI) is correct to question the IACUC on the inconsistent and perhaps overburdensome regulation set forth by this animal care and use program. Permalink : ./index.php?lvl=notice_display&id=107951 [article] Opportunity for Improvement [texte imprimé] / Christina Nascimento . - 2022 . - p. 19-20. Langues : Anglais (eng) in LabAnimal > Vol.22 N°12 (décembre 2022) . - p. 19-20 Mots-clés : communauté scientifique  politique Résumé : This scenario provides several areas for process improvement for the IACUC, researcher and Safety Officer. The use of containment (assumed, biocontainment) may be excessive burden for a commonly used hazardous chemical agent such as tamoxifen. The Principal Investigator (PI) is correct to question the IACUC on the inconsistent and perhaps overburdensome regulation set forth by this animal care and use program. Permalink : ./index.php?lvl=notice_display&id=107951

Réservation

Réserver ce document

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Disponible Disponible

Refinement of the stress-enhanced fear learning model of post-traumatic stress disorder: a behavioral and molecular analysis / Indra A. Van Assche in LabAnimal, Vol.22 N°12 (décembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°12 (décembre 2022) . - p. 6-17 Titre : Refinement of the stress-enhanced fear learning model of post-traumatic stress disorder: a behavioral and molecular analysis Type de document : texte imprimé Auteurs : Indra A. Van Assche ; Mc Stephen Padilla ; Olivia S. R. P. Stupart ; Amy L. Milton Année de publication : 2022 Article en page(s) : p. 6-17 Langues : Anglais (eng) Mots-clés : émotion  extinction  conditionnement de la peur  psychologie  stress post-traumatique  animaux  Traumatologie animale  Comportement animal Résumé : Post-traumatic stress disorder (PTSD) is a debilitating mental health condition for which current treatments have long-term efficacy in 50% of patients. There is a clear need for better understanding of the mechanisms underlying PTSD and the development of new treatment approaches. Analog trauma procedures in animals, such as the stress-enhanced fear learning (SEFL) procedure, can be used to produce behavioral and neurobiological changes that have validity in modeling PTSD. However, by necessity, the modeling of PTSD in animals requires them to potentially experience pain and suffering. Consistent with the ‘3Rs’ (reduction, refinement and replacement) of animal research, this study aimed to determine whether the SEFL procedure can be refined to reduce potential animal pain and suffering while retaining the same behavioral and neurobiological changes. Here we showed that PTSD-relevant changes could be produced in both behavior and the brain of rats that were group- rather than single-housed and that received lower-magnitude electric shocks in the ‘trauma analog’ session. We also varied the number of shock exposures in the trauma analog session, finding SEFL-susceptible and SEFL-resilient populations at all levels of shock exposure, but with greater levels of shock increasing the proportion of rats showing the SEFL-susceptible phenotype. These data demonstrate that the SEFL procedure can be used as an animal analog of PTSD with reduced potential pain and suffering to the animals and that variations in the procedure could be used to generate specific proportions of SEFL-susceptible and SEFL-resilient animals in future studies. Permalink : ./index.php?lvl=notice_display&id=107959 [article] Refinement of the stress-enhanced fear learning model of post-traumatic stress disorder: a behavioral and molecular analysis [texte imprimé] / Indra A. Van Assche ; Mc Stephen Padilla ; Olivia S. R. P. Stupart ; Amy L. Milton . - 2022 . - p. 6-17. Langues : Anglais (eng) in LabAnimal > Vol.22 N°12 (décembre 2022) . - p. 6-17 Mots-clés : émotion  extinction  conditionnement de la peur  psychologie  stress post-traumatique  animaux  Traumatologie animale  Comportement animal Résumé : Post-traumatic stress disorder (PTSD) is a debilitating mental health condition for which current treatments have long-term efficacy in 50% of patients. There is a clear need for better understanding of the mechanisms underlying PTSD and the development of new treatment approaches. Analog trauma procedures in animals, such as the stress-enhanced fear learning (SEFL) procedure, can be used to produce behavioral and neurobiological changes that have validity in modeling PTSD. However, by necessity, the modeling of PTSD in animals requires them to potentially experience pain and suffering. Consistent with the ‘3Rs’ (reduction, refinement and replacement) of animal research, this study aimed to determine whether the SEFL procedure can be refined to reduce potential animal pain and suffering while retaining the same behavioral and neurobiological changes. Here we showed that PTSD-relevant changes could be produced in both behavior and the brain of rats that were group- rather than single-housed and that received lower-magnitude electric shocks in the ‘trauma analog’ session. We also varied the number of shock exposures in the trauma analog session, finding SEFL-susceptible and SEFL-resilient populations at all levels of shock exposure, but with greater levels of shock increasing the proportion of rats showing the SEFL-susceptible phenotype. These data demonstrate that the SEFL procedure can be used as an animal analog of PTSD with reduced potential pain and suffering to the animals and that variations in the procedure could be used to generate specific proportions of SEFL-susceptible and SEFL-resilient animals in future studies. Permalink : ./index.php?lvl=notice_display&id=107959

Réservation

Réserver ce document

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Disponible Disponible

Revisit and Perfect the Policy / Josephine M. Lawrence in LabAnimal, Vol.22 N°12 (décembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°12 (décembre 2022) . - p. 20 Titre : Revisit and Perfect the Policy Type de document : texte imprimé Auteurs : Josephine M. Lawrence ; Felicia D. Duke Boynton Année de publication : 2022 Article en page(s) : p. 20 Langues : Anglais (eng) Mots-clés : communauté scientifique  politique Résumé : Dr. Virale’s predicament presents several questions: what is GEU’s actual policy on housing tamoxifen-treated mice? Is it an IACUC policy or a Safety policy? Is Dr. Virale truly out of compliance, and if so, with which office? These are unanswerable questions in this fictional scenario, but this plot nicely illustrates an actuality most institutions face at some time or another: policies aren’t perfect. Any time a policy isn’t working as intended – it is not providing clear guidance on what is expected or required in the conduct of university business – it should be revisited. Permalink : ./index.php?lvl=notice_display&id=107961 [article] Revisit and Perfect the Policy [texte imprimé] / Josephine M. Lawrence ; Felicia D. Duke Boynton . - 2022 . - p. 20. Langues : Anglais (eng) in LabAnimal > Vol.22 N°12 (décembre 2022) . - p. 20 Mots-clés : communauté scientifique  politique Résumé : Dr. Virale’s predicament presents several questions: what is GEU’s actual policy on housing tamoxifen-treated mice? Is it an IACUC policy or a Safety policy? Is Dr. Virale truly out of compliance, and if so, with which office? These are unanswerable questions in this fictional scenario, but this plot nicely illustrates an actuality most institutions face at some time or another: policies aren’t perfect. Any time a policy isn’t working as intended – it is not providing clear guidance on what is expected or required in the conduct of university business – it should be revisited. Permalink : ./index.php?lvl=notice_display&id=107961

Réservation

Réserver ce document

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Disponible Disponible

The Risk of Hazards, Hypothetically / Lauren Danridge in LabAnimal, Vol.22 N°12 (décembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°12 (décembre 2022) . - p. 18 Titre : The Risk of Hazards, Hypothetically Type de document : texte imprimé Auteurs : Lauren Danridge ; Bill Greer Année de publication : 2022 Article en page(s) : p. 18 Langues : Anglais (eng) Mots-clés : Communauté scientifique  politique Résumé : Dr. Stesso Virale relocated to Great Eastern University (GEU) after working at Great Western University (GWU) for over ten years. Dr. Virale’s research involves a line of transgenic mice that requires treatment with tamoxifen to activate a gene of interest. Permalink : ./index.php?lvl=notice_display&id=107967 [article] The Risk of Hazards, Hypothetically [texte imprimé] / Lauren Danridge ; Bill Greer . - 2022 . - p. 18. Langues : Anglais (eng) in LabAnimal > Vol.22 N°12 (décembre 2022) . - p. 18 Mots-clés : Communauté scientifique  politique Résumé : Dr. Stesso Virale relocated to Great Eastern University (GEU) after working at Great Western University (GWU) for over ten years. Dr. Virale’s research involves a line of transgenic mice that requires treatment with tamoxifen to activate a gene of interest. Permalink : ./index.php?lvl=notice_display&id=107967

Réservation

Réserver ce document

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Disponible Disponible