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Do the right thing for the Elephants and require the PI to submit a protocol / Gayle A. Orner in LabAnimal, Vol.22 N°11 (novembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°11 (novembre 2022) . - p. 32 Titre : Do the right thing for the Elephants and require the PI to submit a protocol Type de document : texte imprimé Auteurs : Gayle A. Orner Année de publication : 2022 Article en page(s) : p. 32 Langues : Anglais (eng) Mots-clés : communauté scientifique  politique Résumé : According to the Animal Welfare Act (AWA), field studies are defined as: “any study conducted on free living animals in their natural habitat, which does not involve an invasive procedure and does not materially alter the behavior of the animal under study”. Permalink : ./index.php?lvl=notice_display&id=107926 [article] Do the right thing for the Elephants and require the PI to submit a protocol [texte imprimé] / Gayle A. Orner . - 2022 . - p. 32. Langues : Anglais (eng) in LabAnimal > Vol.22 N°11 (novembre 2022) . - p. 32 Mots-clés : communauté scientifique  politique Résumé : According to the Animal Welfare Act (AWA), field studies are defined as: “any study conducted on free living animals in their natural habitat, which does not involve an invasive procedure and does not materially alter the behavior of the animal under study”. Permalink : ./index.php?lvl=notice_display&id=107926

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How do you Zoo? / Lauren Danridge in LabAnimal, Vol.22 N°11 (novembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°11 (novembre 2022) . - p. 30-31 Titre : How do you Zoo? Type de document : texte imprimé Auteurs : Lauren Danridge ; Bill Greer Année de publication : 2022 Article en page(s) : p. 30-31 Langues : Anglais (eng) Mots-clés : communauté scientifique  politique Résumé : Dr. Thomas Lauke, a Great Eastern University (GEU) professor, has a National Science Foundation (NSF) grant to develop effective therapies to treat ectoparasites in elephants. Lauke contacted veterinarians who care for elephants at various zoos with Safari Parks across the USA. After he explained his research, they affirmed that they would be willing to assist as collaborators. Permalink : ./index.php?lvl=notice_display&id=107932 [article] How do you Zoo? [texte imprimé] / Lauren Danridge ; Bill Greer . - 2022 . - p. 30-31. Langues : Anglais (eng) in LabAnimal > Vol.22 N°11 (novembre 2022) . - p. 30-31 Mots-clés : communauté scientifique  politique Résumé : Dr. Thomas Lauke, a Great Eastern University (GEU) professor, has a National Science Foundation (NSF) grant to develop effective therapies to treat ectoparasites in elephants. Lauke contacted veterinarians who care for elephants at various zoos with Safari Parks across the USA. After he explained his research, they affirmed that they would be willing to assist as collaborators. Permalink : ./index.php?lvl=notice_display&id=107932

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Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat / Toru Takeo in LabAnimal, Vol.22 N°11 (novembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°11 (novembre 2022) . - p. 8-29 Titre : Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat Type de document : texte imprimé Auteurs : Toru Takeo ; Satohiro Nakao ; Nobuyuki Mikoda ; Katsuma Yamaga ; Ryusei Maeda ; Shuuji Tsuchiyama ; Ena Nakatsukasa ; Naomi Nakagata Année de publication : 2022 Article en page(s) : p. 8-29 Langues : Anglais (eng) Mots-clés : embryologie  ingénierie génétique Résumé : Laboratory rats have been used in biomedical research for over 170 years. Recently, genome editing technology has facilitated the generation of genetically modified rats worldwide. This development has increased the demand for efficient preservation and production of rat resources. Sperm cryopreservation is the most efficient and robust means to archive genetic resources, and this technique reduces the number of animals required for colony management. Previously, we have reported a protocol for rat sperm cryopreservation and in vitro fertilization using frozen–thawed sperm. Here we describe an improved in vitro fertilization protocol to enhance the fertilization rate of cryopreserved sperm in major strains of rats. In this optimized protocol, treatment of frozen–thawed rat sperm with a high concentration of bovine serum albumin (40 mg/ml) results in a high in vitro fertilization rate. This protocol consists of three main steps: preparation of cryopreserved sperm, in vitro fertilization using cryopreserved sperm and embryo transfer. This process takes approximately 1 month to produce live pups from cryopreserved sperm. This protocol can be easily implemented by researchers and technicians with experience in reproductive engineering technology; it can also be used, albeit with some practice, by researchers and technicians who have no experience in reproductive techniques. This sperm cryopreservation and in vitro fertilization protocol for rats will provide an efficient system for the archiving and production of genetically modified rats for the transgenic community. Permalink : ./index.php?lvl=notice_display&id=107952 [article] Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat [texte imprimé] / Toru Takeo ; Satohiro Nakao ; Nobuyuki Mikoda ; Katsuma Yamaga ; Ryusei Maeda ; Shuuji Tsuchiyama ; Ena Nakatsukasa ; Naomi Nakagata . - 2022 . - p. 8-29. Langues : Anglais (eng) in LabAnimal > Vol.22 N°11 (novembre 2022) . - p. 8-29 Mots-clés : embryologie  ingénierie génétique Résumé : Laboratory rats have been used in biomedical research for over 170 years. Recently, genome editing technology has facilitated the generation of genetically modified rats worldwide. This development has increased the demand for efficient preservation and production of rat resources. Sperm cryopreservation is the most efficient and robust means to archive genetic resources, and this technique reduces the number of animals required for colony management. Previously, we have reported a protocol for rat sperm cryopreservation and in vitro fertilization using frozen–thawed sperm. Here we describe an improved in vitro fertilization protocol to enhance the fertilization rate of cryopreserved sperm in major strains of rats. In this optimized protocol, treatment of frozen–thawed rat sperm with a high concentration of bovine serum albumin (40 mg/ml) results in a high in vitro fertilization rate. This protocol consists of three main steps: preparation of cryopreserved sperm, in vitro fertilization using cryopreserved sperm and embryo transfer. This process takes approximately 1 month to produce live pups from cryopreserved sperm. This protocol can be easily implemented by researchers and technicians with experience in reproductive engineering technology; it can also be used, albeit with some practice, by researchers and technicians who have no experience in reproductive techniques. This sperm cryopreservation and in vitro fertilization protocol for rats will provide an efficient system for the archiving and production of genetically modified rats for the transgenic community. Permalink : ./index.php?lvl=notice_display&id=107952

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“Grey” areas of research in a Zoo setting / Julie Marshall in LabAnimal, Vol.22 N°11 (novembre 2022)

Public ISBD [article]  inLabAnimal > Vol.22 N°11 (novembre 2022) . - p. 31 Titre : “Grey” areas of research in a Zoo setting Type de document : texte imprimé Auteurs : Julie Marshall ; Christopher W. Tubbs Année de publication : 2022 Article en page(s) : p. 31 Langues : Anglais (eng) Mots-clés : communauté scientifique  politique Permalink : ./index.php?lvl=notice_display&id=107969 [article] “Grey” areas of research in a Zoo setting [texte imprimé] / Julie Marshall ; Christopher W. Tubbs . - 2022 . - p. 31. Langues : Anglais (eng) in LabAnimal > Vol.22 N°11 (novembre 2022) . - p. 31 Mots-clés : communauté scientifique  politique Permalink : ./index.php?lvl=notice_display&id=107969

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