Dépouillements

Get a look at Galleria / Jim Kling in LabAnimal, 4/2020 (Avril 2020)  

Public ISBD [article]  inLabAnimal > 4/2020 (Avril 2020) . - p. 9-11 Titre : Get a look at Galleria Type de document : texte imprimé Auteurs : Jim Kling Année de publication : 2020 Article en page(s) : p. 9-11 Langues : Anglais (eng) Résumé : As researchers look for more mammalian alternatives, an old moth is learning new tricks. En ligne : https://www.nature.com/articles/s41684-020-0489-1 Permalink : ./index.php?lvl=notice_display&id=88535 [article] Get a look at Galleria [texte imprimé] / Jim Kling . - 2020 . - p. 9-11. Langues : Anglais (eng) in LabAnimal > 4/2020 (Avril 2020) . - p. 9-11 Résumé : As researchers look for more mammalian alternatives, an old moth is learning new tricks. En ligne : https://www.nature.com/articles/s41684-020-0489-1 Permalink : ./index.php?lvl=notice_display&id=88535

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Revue	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Document exclu du prêt - à consulter sur place Exclu du prêt

Know Your Model: A knockout does not always make a null / Susan M. Bello in LabAnimal, 4/2020 (Avril 2020)

Public ISBD [article]  inLabAnimal > 4/2020 (Avril 2020) . - p. 12-13 Titre : Know Your Model: A knockout does not always make a null Type de document : texte imprimé Auteurs : Susan M. Bello ; Michelle N. Perry ; Cynthia L. Smith Année de publication : 2020 Article en page(s) : p. 12-13 Langues : Anglais (eng) Résumé : Creating a null mutation of a gene is a powerful way to examine gene function, but knocking out part of a gene does not always result in a null allele. Permalink : ./index.php?lvl=notice_display&id=88536 [article] Know Your Model: A knockout does not always make a null [texte imprimé] / Susan M. Bello ; Michelle N. Perry ; Cynthia L. Smith . - 2020 . - p. 12-13. Langues : Anglais (eng) in LabAnimal > 4/2020 (Avril 2020) . - p. 12-13 Résumé : Creating a null mutation of a gene is a powerful way to examine gene function, but knocking out part of a gene does not always result in a null allele. Permalink : ./index.php?lvl=notice_display&id=88536

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
Revue	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Document exclu du prêt - à consulter sur place Exclu du prêt

In vivo labeling of epithelial cell–associated antigen passages in the murine intestine / Kathryn A. Knoop in LabAnimal, 4/2020 (Avril 2020)

Public ISBD [article]  inLabAnimal > 4/2020 (Avril 2020) . - p. 21-32 Titre : In vivo labeling of epithelial cell–associated antigen passages in the murine intestine Type de document : texte imprimé Auteurs : Kathryn A. Knoop ; Devesha H. Kulkarni ; Keely G. McDonald ; et al. Année de publication : 2020 Article en page(s) : p. 21-32 Langues : Anglais (eng) Résumé : The intestinal immune system samples luminal contents to induce adaptive immune responses that include tolerance in the steady state and protective immunity during infection. How luminal substances are delivered to the immune system has not been fully investigated. Goblet cells have an important role in this process by delivering luminal substances to the immune system through the formation of goblet cell–associated antigen passages (GAPs). Soluble antigens in the intestinal lumen are transported across the epithelium transcellularly through GAPs and delivered to dendritic cells for presentation to T cells and induction of immune responses. GAPs can be identified and quantified by using the ability of GAP-forming goblet cells to take up fluorescently labeled dextran. Here, we describe a method to visualize GAPs and other cells that have the capacity to take up luminal substances by intraluminal injection of fluorescent dextran in mice under anesthesia, tissue sectioning for slide preparation and imaging with fluorescence microscopy. In contrast to in vivo two-photon imaging previously used to identify GAPs, this technique is not limited by anatomical constraints and can be used to visualize GAP formation throughout the length of the intestine. In addition, this method can be combined with common immunohistochemistry protocols to visualize other cell types. This approach can be used to compare GAP formation following different treatments or changes to the luminal environment and to uncover how sampling of luminal substances is altered in pathophysiological conditions. This protocol requires 8 working hours over 2–3 d to be completed. Permalink : ./index.php?lvl=notice_display&id=88537 [article] In vivo labeling of epithelial cell–associated antigen passages in the murine intestine [texte imprimé] / Kathryn A. Knoop ; Devesha H. Kulkarni ; Keely G. McDonald ; et al. . - 2020 . - p. 21-32. Langues : Anglais (eng) in LabAnimal > 4/2020 (Avril 2020) . - p. 21-32 Résumé : The intestinal immune system samples luminal contents to induce adaptive immune responses that include tolerance in the steady state and protective immunity during infection. How luminal substances are delivered to the immune system has not been fully investigated. Goblet cells have an important role in this process by delivering luminal substances to the immune system through the formation of goblet cell–associated antigen passages (GAPs). Soluble antigens in the intestinal lumen are transported across the epithelium transcellularly through GAPs and delivered to dendritic cells for presentation to T cells and induction of immune responses. GAPs can be identified and quantified by using the ability of GAP-forming goblet cells to take up fluorescently labeled dextran. Here, we describe a method to visualize GAPs and other cells that have the capacity to take up luminal substances by intraluminal injection of fluorescent dextran in mice under anesthesia, tissue sectioning for slide preparation and imaging with fluorescence microscopy. In contrast to in vivo two-photon imaging previously used to identify GAPs, this technique is not limited by anatomical constraints and can be used to visualize GAP formation throughout the length of the intestine. In addition, this method can be combined with common immunohistochemistry protocols to visualize other cell types. This approach can be used to compare GAP formation following different treatments or changes to the luminal environment and to uncover how sampling of luminal substances is altered in pathophysiological conditions. This protocol requires 8 working hours over 2–3 d to be completed. Permalink : ./index.php?lvl=notice_display&id=88537

Exemplaires (1)

Cote	Support	Localisation	Section	Disponibilité
Revue	Revue	Centre de Documentation HELHa Campus Montignies	Armoires à volets	Document exclu du prêt - à consulter sur place Exclu du prêt