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Growth of rare minnows (Gobiocypris rarus) fed different amounts of dietary protein and lipids. / Benli Wu in LabAnimal-Europe, 04/16 (avril 2016)

Public ISBD [article]  inLabAnimal-Europe > 04/16 (avril 2016) . - p. 10-17 Titre : Growth of rare minnows (Gobiocypris rarus) fed different amounts of dietary protein and lipids. Type de document : texte imprimé Auteurs : Benli Wu Année de publication : 2016 Article en page(s) : p. 10-17 Langues : Anglais (eng) Résumé : The rare minnow (Gobiocypris rarus) is a laboratory fish that is commonly used for toxicology research, but there is currently no standard lab diet for this model organism. Certain studies with rare minnows require specialized diets, so there is a need to better understand how manipulating nutrients affects the development and growth of this fish. We conducted two separate dose-response experiments to determine the effect of different levels of dietary protein or dietary lipids on the growth of juvenile rare minnows over 60 d. We measured growth rates and food intake over two periods of time: the first 20 or 30 d of diet consumption and the entire 60 d of each experiment. We found that different levels of dietary protein or dietary lipids produced significantly different growth rates during both the early phase and the entire duration of the study. Among experimental protein-variable diets, those with intermediate levels of dietary protein (around 35.2%) produced the highest growth rate. Among experimental lipid-variable diets, those with intermediate levels of dietary lipids (around 7.6%) produced the highest growth rate. Over all periods of both experiments, however, the control diet of bloodworms generally produced the highest growth rate that matched or exceeded that of any experimental diet. These results can guide investigators when using rare minnows in research, particularly when using custom and standardized diets. Permalink : ./index.php?lvl=notice_display&id=76495 [article] Growth of rare minnows (Gobiocypris rarus) fed different amounts of dietary protein and lipids. [texte imprimé] / Benli Wu . - 2016 . - p. 10-17. Langues : Anglais (eng) in LabAnimal-Europe > 04/16 (avril 2016) . - p. 10-17 Résumé : The rare minnow (Gobiocypris rarus) is a laboratory fish that is commonly used for toxicology research, but there is currently no standard lab diet for this model organism. Certain studies with rare minnows require specialized diets, so there is a need to better understand how manipulating nutrients affects the development and growth of this fish. We conducted two separate dose-response experiments to determine the effect of different levels of dietary protein or dietary lipids on the growth of juvenile rare minnows over 60 d. We measured growth rates and food intake over two periods of time: the first 20 or 30 d of diet consumption and the entire 60 d of each experiment. We found that different levels of dietary protein or dietary lipids produced significantly different growth rates during both the early phase and the entire duration of the study. Among experimental protein-variable diets, those with intermediate levels of dietary protein (around 35.2%) produced the highest growth rate. Among experimental lipid-variable diets, those with intermediate levels of dietary lipids (around 7.6%) produced the highest growth rate. Over all periods of both experiments, however, the control diet of bloodworms generally produced the highest growth rate that matched or exceeded that of any experimental diet. These results can guide investigators when using rare minnows in research, particularly when using custom and standardized diets. Permalink : ./index.php?lvl=notice_display&id=76495

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Collection of untainted urinary specimens from the bladder of an anesthetized rabbit. / Patrick A. Walsh in LabAnimal-Europe, 04/16 (avril 2016)

Public ISBD [article]  inLabAnimal-Europe > 04/16 (avril 2016) . - p. 20-22 Titre : Collection of untainted urinary specimens from the bladder of an anesthetized rabbit. Type de document : texte imprimé Auteurs : Patrick A. Walsh Année de publication : 2016 Article en page(s) : p. 20-22 Langues : Anglais (eng) Mots-clés : Anesthesia/veterinary  Animals  Catheters, Indwelling/veterinary  Male  Rabbits*  Urinary Bladder*  Urinary Catheterization/methods  Urinary Catheterization/veterinary*  Urine Specimen  Collection/methods  Urine Specimen Collection/veterinary* Résumé : It is difficult to collect untainted urine specimens over short intervals of time during renal studies with rabbits. This is because both the ureters and the bladder of this species are relatively friable and minor manipulation can easily cause intraluminal bleeding. We have developed and refined an effective technique and protocol for placing an indwelling urinary bladder catheter into an anesthetized rabbit. The procedure is easy to perform and completely effective and reliable, allowing high-quality urinary specimens to be collected at intervals of 15-20 min over a period of 3-4 hours during a study of acute metabolic acidosis. Permalink : ./index.php?lvl=notice_display&id=76496 [article] Collection of untainted urinary specimens from the bladder of an anesthetized rabbit. [texte imprimé] / Patrick A. Walsh . - 2016 . - p. 20-22. Langues : Anglais (eng) in LabAnimal-Europe > 04/16 (avril 2016) . - p. 20-22 Mots-clés : Anesthesia/veterinary  Animals  Catheters, Indwelling/veterinary  Male  Rabbits*  Urinary Bladder*  Urinary Catheterization/methods  Urinary Catheterization/veterinary*  Urine Specimen  Collection/methods  Urine Specimen Collection/veterinary* Résumé : It is difficult to collect untainted urine specimens over short intervals of time during renal studies with rabbits. This is because both the ureters and the bladder of this species are relatively friable and minor manipulation can easily cause intraluminal bleeding. We have developed and refined an effective technique and protocol for placing an indwelling urinary bladder catheter into an anesthetized rabbit. The procedure is easy to perform and completely effective and reliable, allowing high-quality urinary specimens to be collected at intervals of 15-20 min over a period of 3-4 hours during a study of acute metabolic acidosis. Permalink : ./index.php?lvl=notice_display&id=76496

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Methods for measuring pain in laboratory animals. / Dustin M. Graham in LabAnimal-Europe, 04/16 (avril 2016)

Public ISBD [article]  inLabAnimal-Europe > 04/16 (avril 2016) . - p. 23-25 Titre : Methods for measuring pain in laboratory animals. Type de document : texte imprimé Auteurs : Dustin M. Graham Année de publication : 2016 Article en page(s) : p. 23-25 Langues : Anglais (eng) Mots-clés : Animals  Animals, Laboratory*  Behavior, Animal  Facial Expression  Movement/physiology  Nociception  Pain/physiopathology  Pain Measurement/methods* Résumé : Pain is one of the most important variables that members of the lab animal science community try to control and minimize, and it is a critical topic of research in clinical and basic sciences. Objective measurements of pain severity, especially in non-verbal animals that are used as disease models, can be difficult to obtain, but several developments in behavioral neuroscience are making the measurement of pain more consistent, automated and accurate. Permalink : ./index.php?lvl=notice_display&id=76497 [article] Methods for measuring pain in laboratory animals. [texte imprimé] / Dustin M. Graham . - 2016 . - p. 23-25. Langues : Anglais (eng) in LabAnimal-Europe > 04/16 (avril 2016) . - p. 23-25 Mots-clés : Animals  Animals, Laboratory*  Behavior, Animal  Facial Expression  Movement/physiology  Nociception  Pain/physiopathology  Pain Measurement/methods* Résumé : Pain is one of the most important variables that members of the lab animal science community try to control and minimize, and it is a critical topic of research in clinical and basic sciences. Objective measurements of pain severity, especially in non-verbal animals that are used as disease models, can be difficult to obtain, but several developments in behavioral neuroscience are making the measurement of pain more consistent, automated and accurate. Permalink : ./index.php?lvl=notice_display&id=76497

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