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Mice on the move / Charlotte Harrison in LabAnimal, Vol. 21 N°10 (October 2021)
[article]
Titre : Mice on the move Type de document : texte imprimé Auteurs : Charlotte Harrison Année de publication : 2021 Article en page(s) : p. 37-40 Note générale : DOI: 10.1038/s41684-021-00829-5 Langues : Anglais (eng) Mots-clés : Animal Welfare Animals Animals, Laboratory Mice Transportation / economics Résumé : Animal welfare concerns, reduced flight options and increased costs are pushing researchers to consider shipping frozen sperm and embryos rather than live mice. Permalink : ./index.php?lvl=notice_display&id=96700
in LabAnimal > Vol. 21 N°10 (October 2021) . - p. 37-40[article] Mice on the move [texte imprimé] / Charlotte Harrison . - 2021 . - p. 37-40.
DOI: 10.1038/s41684-021-00829-5
Langues : Anglais (eng)
in LabAnimal > Vol. 21 N°10 (October 2021) . - p. 37-40
Mots-clés : Animal Welfare Animals Animals, Laboratory Mice Transportation / economics Résumé : Animal welfare concerns, reduced flight options and increased costs are pushing researchers to consider shipping frozen sperm and embryos rather than live mice. Permalink : ./index.php?lvl=notice_display&id=96700 Réservation
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DisponibleKnow Your Model: A brief history of making mutant mouse genetic models / Susan M. Bello in LabAnimal, Vol. 21 N°11 (November 2021)
[article]
Titre : Know Your Model: A brief history of making mutant mouse genetic models Type de document : texte imprimé Auteurs : Susan M. Bello ; Michelle N. Perry ; Cynthia L. Smith Année de publication : 2021 Article en page(s) : p. 31-34 Langues : Anglais (eng) Mots-clés : Animals Mice Models, Genetic* Résumé : For over a century, researchers have used mice as models and adapted many new methods to create novel mutations in them. In the past 100+ years, we have gone from breeding strains for selected traits to inducing random mutations throughout the genome to creating designer alleles with multiple functions. Each method offers opportunities and challenges for researchers as they try to address specific research questions with mouse models. Permalink : ./index.php?lvl=notice_display&id=98140
in LabAnimal > Vol. 21 N°11 (November 2021) . - p. 31-34[article] Know Your Model: A brief history of making mutant mouse genetic models [texte imprimé] / Susan M. Bello ; Michelle N. Perry ; Cynthia L. Smith . - 2021 . - p. 31-34.
Langues : Anglais (eng)
in LabAnimal > Vol. 21 N°11 (November 2021) . - p. 31-34
Mots-clés : Animals Mice Models, Genetic* Résumé : For over a century, researchers have used mice as models and adapted many new methods to create novel mutations in them. In the past 100+ years, we have gone from breeding strains for selected traits to inducing random mutations throughout the genome to creating designer alleles with multiple functions. Each method offers opportunities and challenges for researchers as they try to address specific research questions with mouse models. Permalink : ./index.php?lvl=notice_display&id=98140 Réservation
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DisponibleOral application of clozapine-N-oxide using the micropipette-guided drug administration (MDA) method in mouse DREADD systems / Sina M Schalbetter in LabAnimal, 04/21 (Avril 2021 vol. 21 N°4)
[article]
Titre : Oral application of clozapine-N-oxide using the micropipette-guided drug administration (MDA) method in mouse DREADD systems Type de document : texte imprimé Auteurs : Sina M Schalbetter ; Flavia S Mueller ; Joseph Scarborough ; et al. Année de publication : 2021 Article en page(s) : p.14-27 Note générale : DOI: 10.1038/s41684-021-00723-0 Langues : Anglais (eng) Mots-clés : Animals Brain Clozapine Designer Drugs Mice Neurons Oxides Résumé : The designer receptor exclusively activated by designer drugs (DREADD) system is one of the most widely used chemogenetic techniques to modulate the activity of cell populations in the brains of behaving animals. DREADDs are activated by acute or chronic administration of their ligand, clozapine-N-oxide (CNO). There is, however, a current lack of a non-invasive CNO administration technique that can control for drug timing and dosing without inducing substantial distress for the animals. Here, we evaluated whether the recently developed micropipette-guided drug administration (MDA) method, which has been used as a non-invasive and minimally stressful alternative to oral gavages, may be applied to administer CNO orally to activate DREADDs in a dosing- and timing-controlled manner. Unlike standard intraperitoneal injections, administration of vehicle substances via MDA did not elevate plasma levels of the major stress hormone, corticosterone, and did not attenuate exploratory activity in the open field test. At the same time, however, administration of CNO via MDA or intraperitoneally was equally efficient in activating hM3DGq-expressing neurons in the medial prefrontal cortex, as evident by time-dependent increases in mRNA levels of neuronal immediate early genes (cFos, Arc and Zif268) and cFos-immunoreactive neurons. Compared to vehicle given via MDA, oral administration of CNO via MDA was also found to potently increase locomotor activity in mice that express hM3DGq in prefrontal neurons. Taken together, our study confirms the effectiveness of CNO given orally via MDA and provides a novel method for non-stressful, yet well controllable CNO treatments in mouse DREADD systems. Permalink : ./index.php?lvl=notice_display&id=94499
in LabAnimal > 04/21 (Avril 2021 vol. 21 N°4) . - p.14-27[article] Oral application of clozapine-N-oxide using the micropipette-guided drug administration (MDA) method in mouse DREADD systems [texte imprimé] / Sina M Schalbetter ; Flavia S Mueller ; Joseph Scarborough ; et al. . - 2021 . - p.14-27.
DOI: 10.1038/s41684-021-00723-0
Langues : Anglais (eng)
in LabAnimal > 04/21 (Avril 2021 vol. 21 N°4) . - p.14-27
Mots-clés : Animals Brain Clozapine Designer Drugs Mice Neurons Oxides Résumé : The designer receptor exclusively activated by designer drugs (DREADD) system is one of the most widely used chemogenetic techniques to modulate the activity of cell populations in the brains of behaving animals. DREADDs are activated by acute or chronic administration of their ligand, clozapine-N-oxide (CNO). There is, however, a current lack of a non-invasive CNO administration technique that can control for drug timing and dosing without inducing substantial distress for the animals. Here, we evaluated whether the recently developed micropipette-guided drug administration (MDA) method, which has been used as a non-invasive and minimally stressful alternative to oral gavages, may be applied to administer CNO orally to activate DREADDs in a dosing- and timing-controlled manner. Unlike standard intraperitoneal injections, administration of vehicle substances via MDA did not elevate plasma levels of the major stress hormone, corticosterone, and did not attenuate exploratory activity in the open field test. At the same time, however, administration of CNO via MDA or intraperitoneally was equally efficient in activating hM3DGq-expressing neurons in the medial prefrontal cortex, as evident by time-dependent increases in mRNA levels of neuronal immediate early genes (cFos, Arc and Zif268) and cFos-immunoreactive neurons. Compared to vehicle given via MDA, oral administration of CNO via MDA was also found to potently increase locomotor activity in mice that express hM3DGq in prefrontal neurons. Taken together, our study confirms the effectiveness of CNO given orally via MDA and provides a novel method for non-stressful, yet well controllable CNO treatments in mouse DREADD systems. Permalink : ./index.php?lvl=notice_display&id=94499 Réservation
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DisponibleConstructing an inexpensive and versatile homemade rodent treadmill / Steven Bouganim in LabAnimal, 04/17 (avril 2017)
[article]
Titre : Constructing an inexpensive and versatile homemade rodent treadmill Type de document : texte imprimé Auteurs : Steven Bouganim ; Andreas Bergdahl Année de publication : 2017 Article en page(s) : p. 16-19 Langues : Anglais (eng) Mots-clés : Animals Equipment Design Mice Physical Conditioning Animal/instrumentation Rats Permalink : ./index.php?lvl=notice_display&id=66487
in LabAnimal > 04/17 (avril 2017) . - p. 16-19[article] Constructing an inexpensive and versatile homemade rodent treadmill [texte imprimé] / Steven Bouganim ; Andreas Bergdahl . - 2017 . - p. 16-19.
Langues : Anglais (eng)
in LabAnimal > 04/17 (avril 2017) . - p. 16-19
Mots-clés : Animals Equipment Design Mice Physical Conditioning Animal/instrumentation Rats Permalink : ./index.php?lvl=notice_display&id=66487 Réservation
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DisponibleDetection of mouse viruses in patient-derived xenografts / Alexandra Le Bras in LabAnimal, 06/21 (June 2021 N°6)
[article]
Titre : Detection of mouse viruses in patient-derived xenografts Type de document : texte imprimé Auteurs : Alexandra Le Bras Année de publication : 2021 Article en page(s) : p. 6-7 Note générale : DOI: 10.1038/s41684-021-00761-8 Langues : Anglais (eng) Mots-clés : Animals Disease Models, Animal Heterografts Mice Viruses En ligne : https://www.nature.com/articles/s41684-021-00761-8 Permalink : ./index.php?lvl=notice_display&id=94505
in LabAnimal > 06/21 (June 2021 N°6) . - p. 6-7[article] Detection of mouse viruses in patient-derived xenografts [texte imprimé] / Alexandra Le Bras . - 2021 . - p. 6-7.
DOI: 10.1038/s41684-021-00761-8
Langues : Anglais (eng)
in LabAnimal > 06/21 (June 2021 N°6) . - p. 6-7
Mots-clés : Animals Disease Models, Animal Heterografts Mice Viruses En ligne : https://www.nature.com/articles/s41684-021-00761-8 Permalink : ./index.php?lvl=notice_display&id=94505 Réservation
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DisponibleHow much access to laboratories should veterinarians have? / Jerald Silverman in LabAnimal, 03/17 (mars 2017)
PermalinkThe impact of COVID-19 lockdowns on the genetic integrity of your mouse colonies / Natalia Moncaut in LabAnimal, Vol. 21 N°12 (December 2021)
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